Efficient scarless skin regeneration enabled by loading micronized amnion in a bioinspired adhesive wound dressing

Complete skin reconstruction is a hierarchical, physiological assembly process involving healing of the epidermis, dermis, vasculature, nerves, and cutaneous appendages. To date, few works have reported complete regeneration, particularly lacking vascular structures hair follicles after full defects. In this study, hydrogel derived from secretion Andrias davidianus (SSAD) that features adhesiveness was used as bioactive scaffold to load micronized amnion (MA). The SSAD found promote migration proliferation stem cells human keratinocytes, well inhibit their apoptosis in vitro. rat full-skin defect model, regeneration appendages observed at wound area, achieving scarless healing. Transcriptome analyses further validated could positively regulate cell migration, proliferation, differentiation. These functions might be attributed abundant growth factors present SSAD. Synergized by delivery MA, loaded with MA achieve significantly better effect than or alone, providing simple yet highly effective means obtain complete, suggesting favorable potential for clinical translation.